orals 5th Asia-Pacific NMR Symposium 2013

The design of targetable RNA-binding proteins (#10)

Joel Mackay 1 , Marylene Vandevenne 1 , Mitchell O'Connell 1 , Stephanie Helder 1 , David Segal 2
  1. School of Molecular Bioscience, University of Sydney, Darlington, NSW, Australia
  2. UC Davis, Davis, CA, USA

In recent years, many diverse classes of RNA have been discovered, and it has become clear that these species play a wide range of roles in cellular metabolism. Protein tools to track and manipulate RNA would be of great use both in research and potentially as therapeutics. We are currently testing the hypothesis that zinc-finger domains can act as a suitable scaffold with which to design RNA-binding proteins with programmable specificity.1  I will describe our progress to date in this area, including our efforts to structurally and functionally characterize the new protein domains that we have designed.

  1. O'Connell et al. (2012) Modular assembly of RanBP2-type zinc finger domains to target single-stranded RNA. Angew. Chem. 51, 5371-5