posters 5th Asia-Pacific NMR Symposium 2013

Solution Structure of Crustacean Hyperglycemic Hormone-like (CHHL) from Scylla olivacea (#111)

Yun-Ru Chen 1 , Kumar M Sriramoju 1 , Chi-Ying Lee 2 , Ping-Chiang Lyu 1
  1. Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan
  2. Department of Biology, National Changhua University of Education, Changhua, Taiwan

Scylla olivacea Crustacean Hyperglycemic Hormone-like (CHHL), a small neuropeptide is synthesized and released by the X-organ sinus gland complex of eyestalk from mud crab (Scylla olivaces). This CHHL, which contains 74 amino acids and 3 highly conserved disulfide bonds, belongs to the Crustacean hyperglycemic hormone (CHH) family. The CHH family neuropeptides have diverse biological functions such as carbohydrate metabolism and molt-inhibiting. However, little information of biological function for CHHL is reported. Based on the biological and molecular properties of the CHH family are clustered into two sub-groups, CHH and MIH. Only the solution structure of Molt-inhibiting hormone (MIH) has been solved. To explore the structure-function relationship of CHHL, we have created recombinant CHHL and solved the solution structure of CHHL from Scylla olivacea by employing heteronuclaer NMR spectroscopy.