orals 5th Asia-Pacific NMR Symposium 2013

Magnetizing Proteins for NMR (#3)

Gottfried Otting 1
  1. Research School of Chemistry, Australian National University, Canberra, Australia

Paramagnetic probes present outstanding opportunities for structural biology studies by nuclear magnetic resonance (NMR) spectroscopy. In particular, pseudocontact shifts (PCS) from lanthanide labeled proteins provide unique long-range restraints that help to define the structures of proteins, protein-protein complexes, and protein-ligand complexes [1,2]. The value of PCSs will be illustrated with a range of applications, including resonance assignments and structure determinations of proteins and protein-ligand complexes, and the merits of different paramagnetic tags will be discussed [3, 4].

  1. G. Otting (2008) J. Biomol. NMR 42, 1-9.
  2. H. Yagi et al. (2013) Structure 21, 883-890.
  3. X.C Su and G. Otting (2010) J. Biomol. NMR 46, 101-112.
  4. D. Shishmarev and G. Otting (2013) J. Biomol. NMR 56, 203-216.