Scylla olivacea Crustacean Hyperglycemic Hormone-like (CHHL), a small neuropeptide is synthesized and released by the X-organ sinus gland complex of eyestalk from mud crab (Scylla olivaces). This CHHL, which contains 74 amino acids and 3 highly conserved disulfide bonds, belongs to the Crustacean hyperglycemic hormone (CHH) family. The CHH family neuropeptides have diverse biological functions such as carbohydrate metabolism and molt-inhibiting. However, little information of biological function for CHHL is reported. Based on the biological and molecular properties of the CHH family are clustered into two sub-groups, CHH and MIH. Only the solution structure of Molt-inhibiting hormone (MIH) has been solved. To explore the structure-function relationship of CHHL, we have created recombinant CHHL and solved the solution structure of CHHL from Scylla olivacea by employing heteronuclaer NMR spectroscopy.